当前位置 :
帮我做一题分子生物学题目?我会再申几个号给你加分的!YouhaveisolatedanewstrainofanE.coli-likebacteriafromhospitalpatients.Uponsequencingthegenome,youdiscoverthatthisbacteriahasagroupofabout30genes,absent
1人问答
八字精批流年运程八字合婚八字起名
问题描述:

帮我做一题分子生物学题目?我会再申几个号给你加分的!

YouhaveisolatedanewstrainofanE.coli-likebacteriafromhospitalpatients.Uponsequencingthegenome,youdiscoverthatthisbacteriahasagroupofabout30genes,absentfromthestandardlabstrains,thatyoususpectareinvolvedinpathogenesis(发病机理).Youinitiateaprojecttounderstandtheexpressionandfunctionofthesegenes.

BycomparingmRNAsizeswiththeDNAsequence,yourealizethatoneofthesegenes,pukC,appearstocontainanintron.Sincebacterialackthespliceosomecomponentsfoundinhighercells,youhypothesizethatyouhaveisolatedanewself-splicingintron.

SequenceanalysisofthepukCintronfailedtorevealclearhomologytoeitherthegroupIorgroup2self-splicingintronfamilies.

TostudythemechanismofsplicingyouclonethepukCgeneontoaplasmidbehindagoodpromoter.ThisconstructallowsyoutosynthesizethepukCpre-mRNAusinginvitrotranscription.Whenyouruntheproductsofthisfirsttranscriptionexperimentonagel,youseeRNAmoleculeswithlengthssuggestingthatsplicingisoccurring(althoughveryslowly)duringthetranscriptionreactions.

Question1:Giventhisresult,suggestamodificationoftheinvitro

reactionusedabovethatcouldgiveaquickindicationofwhetherthepukCintronusesamechanismsimilartothegroupIorsimilartothegroupIIintrons.

Question2:Nowdescribeamoredefinitiveexperimentthatwillallow

youtoshowthatthepukCintronuseseitheragroupIorgroupII-like

mechanism.Whatresultsdoyouexpecttoobserveforeachofthetwo

mechanisms?

可以用汉语吗?我看不懂这个!

戴菡回答:
  THEANSWEROFQUESTION1:GroupIintronsrequireaGnucleotideornucleoside,whicharearoundintheoriginalexperiment.ModifytheoriginalexperimentbyisolatingtheRNAawayfromtherNTPsand...
八字精批 八字合婚 八字起名 八字财运 2024运势 测终身运 姓名详批 结婚吉日
已出生未出生
最新更新
PC端 | 移动端 | mip端
字典翻译(zidianfy.com)汇总了汉语字典,新华字典,成语字典,组词,词语,在线查字典,中文字典,英汉字典,在线字典,康熙字典等等,是学生查询学习资料的好帮手,是老师教学的好助手。
声明:本网站尊重并保护知识产权,根据《信息网络传播权保护条例》,如果我们转载的作品侵犯了您的权利,请在一个月内通知我们,我们会及时删除。
电话:  邮箱:
Copyright©2009-2021 字典翻译 zidianfy.com 版权所有 闽ICP备2022014709号-7
lyric 頭條新聞
复制重新加载
原创不易,您的支持将成为鼓励我的动力
《帮我做一题分子生物学题目?我会再申几个号给你加分的!YouhaveisolatedanewstrainofanE.coli-likebacteriafromhospitalpatients.Uponsequencingthegenome,youdiscoverthatthisbacteriahasagroupofabout30genes,absent|小学英语问答-字典翻译问答网》
1、付费复制方式
支付宝付费后即可复制当前文章
限时特价:5.99元
原价:20元
打开支付页
2、微信付费复制方式
微信扫码付费后即可复制当前文章
限时特价:5.99元
原价:20元